Laboratoire d'endocrinologie circadienne

Responsable: Pre Charna DIBNER 

• Division of Endocrinology, Nutrition and Therapeutic Education for Patient
• Département de Médicine
• Department of Cell Physiology and Metabolism
• Faculty of Medicine, University of Geneva

Address: 
Centre Médical Universitaire D05.2147a 
Dibner Group, PHYM
Rue Michel-Servet, 1
CH-1211 Geneva 4, Switzerland
Phone:  +41 22 3795934
Email: Charna.Dibner@hcuge.ch

Activités de recherche

Le but de la recherche menée par ce groupe est d'explorer le lien entre les horloges circadiennes mammifères et le métabolisme. L'équipe de recherche s'intéresse en particulier à l'importance physiologique d'une horloge pancréatique et son rôle dans les maladies métaboliques et le diabète de type 2 au travers de trois projets:

1. Rôle des horloges cellulaires sur la fonction des cellules béta pancréatiques: l’enregistrement de la bioluminescence dans les cellules vivantes est employé pour analyser l’expression des gènes et leur fonction dans des conditions physiologiques et de rythme circadien aboli
2. Rôle des horloges cellulaires de la souris sur l’homéostasie du glucose: l’enregistrement de la bioluminescence en temps réel permet d’étudier la contribution des horloges biologiques à la sécrétion d’insuline et l’homéostasie du glucose dans des conditions physiologiques et de diabète de type 2
3. Etude des horloges circadiennes humaines: à travers les rythmes circadiens de cellules provenant de biopsies cutanées, la fonction des horloges est comparée chez des sujets en bonne santé et diabétiques

Thèses de doctorat

• Dr Laurent Perrin, 2018
• Dr Anne-Marie Makhlouf, 2017

Group members

• Pr. Simona Chera, University of Bergen, visiting professor
• Mrs. Oksana Fiammingo, PhD student
• Dr. Luiza Ghila, Scientific collaborator
• M. Dylan Gendre, Assitant de recherche ; Dylan.Gendre@unige.ch
• Dr. Volodymyr Petrenko, Maitre-Assistant ; volodymyr.petrenko@unige.ch
• Dr. Flore Sinturel, Maitre-Assistant ; flore.sinturel@unige.ch
• Mrs. Ana-Lucia Del Solar Reyes, MSc student ; Ana-Lucia.Del-Solar@etu.unige.ch

Past group members

• Dr. Anne-Marie Makhlouf, former PhD student, currently senior collaborator in Nutrition Unit, HUG
• Dr. Camille Saini, postdoc in our lab, currently Clinical Coordinator, Epidemiology Unit, HUG
• Dr. Laurianne Giovannoni, scientific collaborator in our lab, currently MD at Paediatric Service, HUG
• Dr. Zhanna Chitikova, guest scientist in our lab (volunteer), currently postdoc at Dpt of Molecular Biology, Faculty of Science, University of Geneva  
• Dr. Pamela Pulimeno, postdoc in our lab, currently senior postdoc at Harvard Medical School, Boston, US
• Dr. Ursula Loizides-Mangold, Scientific Collaborator
• Mr. Laurent Perrin, PhD student

Selected recent publications by the group

2020

Articles scientifiques

• Sinturel F, Petrenko V, Dibner C. Circadian clocks make metabolism run. Journal of Molecular Biology 2020;432(12):3680-3699.
• Petrenko V, Gandasi NR, Sage D, Tengholm A, Barg S, Dibner C. In pancreatic islets from type 2 diabetes patients, the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis. Proceedings of the National Academy of Sciences 2020;117(5):2484-2495.
• Somm E, Montandon SA, Loizides-Mangold UE, Gaia N, Lazarevic V, De Vito C, Perroud EN, Piallat M-L, et al. The GLP-1R agonist liraglutide limits hepatic lipotoxicity and inflammatory response in mice fed a methionine-choline deficient diet. Translational Research 2020

Directions de master

• Del Solar Reyes AL. Circadian expression of core clock and functional genes is altered in porcine smooth muscle cell populations derived from atheroma-prone aortic tissue. 2020

2019

Articles scientifiques

• Sinturel F, Makhlouf A-M, Meyer P, Tran C, Pataky Z, Golay A, Rey G, Howald C, et al. Cellular circadian period length inversely correlates with HbA1c levels in individuals with type 2 diabetes. Diabetologia 2019;62(8):1453-1462.
• Montandon S, Somm E, Loizides-Mangold U, De Vito C, Dibner C, Jornayvaz F. Multi-technique comparison of atherogenic and MCD NASH models highlights changes in sphingolipid metabolism. Scientific Reports 2019;9(1):16810.
• Dibner C. The importance of being rhythmic: living in harmony with your body clocks. Acta Physiologica 2019:e13281.
• Sadowski Veuthey MSD, Petrenko V, Meyer P, Pusztaszeri M, Brulhart-Meynet M-C, Heddad Masson M, Triponez F, Philippe J, et al. Validation of molecular biomarkers for preoperative diagnostics of human papillary thyroid carcinoma in fine needle aspirates. Gland Surgery 2019;8(Suppl 2):S62-S76.

Directions de thèse

• Morele Giovannoni L. Les horloges circadiennes des cellules α- et β- pancréatiques ont des propriétés moléculaires et un impact différent sur la sécrétion hormonale de l’îlot et l’expression des gènes. 2019

2018 

Articles scientifiques

• Dibner C, Schibler U. Body clocks: Time for the Nobel Prize. Acta Physiologica 2018;222(2).
• Dibner C. Cell-specific resetting of mouse islet cellular clocks by glucagon, glucagon-like peptide 1 and somatostatin. Acta Physiologica 2018;222(4):e13021.
• Sadowski Veuthey MSD, Pusztaszeri M, Brulhart-Meynet M-C, Petrenko V, De Vito C, Sobel J, Delucinge C, Kebebew E, et al. Identification of Differential Transcriptional Patterns in Primary and Secondary Hyperparathyroidism. Journal of Clinical Endocrinology and Metabolism 2018
• Petrenko V, Philippe J, Dibner C. Time zones of pancreatic islet metabolism. Diabetes, Obesity and Metabolism 2018;20(Suppl 2):116-126.
• Perrin L, Loizides-Mangold U, Chanon S, Gobet C, Hulo N, Isenegger L, Weger BD, Migliavacca Voeffray EL, et al. Transcriptomic analyses reveal rhythmic and CLOCK-driven pathways in human skeletal muscle. eLife 2018;7:e34114.

2017

Original articles

• Jacovetti C., Rodriguez A., Gattesco S., Petrenko V., Saini C., Dibner C., Regazzi R. “MicroRNAs modulate core-clock gene expression in pancreatic islets during early postnatal lifes.” Research article, Diabetologia IF 6.487
• Chanon S., Durand C., Vieille-Marchiset A., Robert M., Dibner C., Simon C., Lefai E. “Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes.” J Vis Exp IF 1.1
• 3. Petrenko V., Gosmain Y., and Dibner C. “High-resolution recording of circadian oscillator features in primary cultures of mouse a- and b-cells.” Original article, accepted for publication. Frontiers in Endocrinology IF: to be attributed in 2017
• 4. Petrenko V., Saini C., Giovannoni L., Gobet C., Sage D., Unser M., Heddad-Mason M., Gu G., Bosco D., Gachon F., Philippe J., Dibner C. “Pancreatic a- and b-cellular clocks impact differentially on hormone secretion and gene transcription due to their distinct molecular properties.” Genes and Development 2017, Feb 15;31(4):383-398. IF 11.7, 6 citations
• Research highlight on this article: Haspel J. “Clocks stop sugar shock” Science Translational Medicine

Review articles

• Petrenko V. and Dibner C. “Circadian orchestration of insulin and glucagon release.” Invited featured article, accepted for publication in Cell Cycle IF 4.56
• Gachon F., Loizides-Mangold U., Petrenko V. and Dibner C. “Glucose homeostasis: regulation by peripheral circadian clocks in rodents and humans.” Endocrinology 2017, Mar 15. doi: 10.1210/en.2017-00218. IF 4.2
• Dibner C., Sadowski S., Triponez F. and Philippe J. “The search for preoperative biomarkers for thyroid carcinoma: application of the thyroid circadian clock properties” Invited perspective article, accepted for publication. Biomark Med. 2017, Mar;11(3):285-293 IF 2.3

Directions de thèse

• Perrin L. Circadian regulation of human skeletal muscle function in physiology and insulin resistance. 2017

2016

Original articles

• Petrenko V., Saini C., Perrin L., Dibner C. “Parallel measurement of circadian clock gene expression and hormone secretion in human primary cell cultures.” J Vis Exp., 2016, Nov 11;(117). IF1.1, 2 citations
• Makhlouf A.-M., Chitikova Z., Pusztaszeri M., Bercy M., Delucinge-Vivier C., Triponez F., Meyer P., Philippe J., and Dibner C. “Identification of CHEK1, SLC26A4, c-KIT, TPO and TG as new biomarkers for human follicular thyroid carcinoma diagnostics” Oncotarget 2016, Jun 18. doi: 10.18632. IF 6.37, 6 citations
• Loizides-Mangold U., Koren-Gluzer M., Skarupelova S., Makhlouf A.-M., Hayek T., Aviram M., Dibner C. “Paraoxonase 1 (PON1) and pomegranate influence circadian gene expression and period length.” Chronobiology International, IF 3.3, 1 citation
• Saini C., Petrenko V., Pulimeno P., Giovannoni L., Berney T., Hebrok M., Howald C., Dermitzakis E., and Dibner C. “A functional circadian clock is required for proper insulin secretion by human pancreatic islet cells.” Diabetes, Obesity and Metabolism 2016 Apr;18(4):355-65. IF 6.4, 15 citations

PhD Thesis Defense

• Dr. Anne-Marie-Makhlouf, Study of the circadian rhythm in healthy volunteers, obese and type 2 diabetic patients. 2016

2015

Original articles

• Perrin L., Loizides-Mangold U., Skarupelova S., Pulimeno P., Chanon S., Robert M., Bouzakri K., Moudoux C., Roux-Lombard P., Vidal H., Lefai E. and Dibner C. “Human skeletal myotubes display a cell-autonomous circadian clock implicated in basal myokine secretion.” Molecular Metabolism 2015, 4 (11), 834-845. IF 5.5, 25 citations
• Chitikova Z., Pusztaszeri M., Makhlouf A.-M., Bercy M., Delucinge-Vivier C., Triponez F., Meyer P., Philippe J., and Dibner C.  “Identification of new biomarkers for human papillary thyroid carcinoma employing NanoString analysis.” Oncotarget 2015 May 10;6(13):10978-93. IF 6.4, 12 citations

Review articles

• Dibner C. and Schibler U. “A pancreatic clock times insulin release” Science 2015, 350 (6261), 628-629. IF 33.6, 5 citations
• Dibner C. and Gachon F. “Circadian dysfunction and obesity: is Leptin the missing link?” Cell Metabolism 2015 Vol. 22(3) 359-360. IF 17.6
• Saini C., Brown S. and Dibner C. “Skin fibroblast clocks: applications to studying human circadian phenotypes in physiology and pathophysiology.”  Frontiers in Neurology 2015 May 13;6:95. IF 3.6, 28 citations
• Dibner C., Schibler U. “Circadian clocks: roles in endocrinology and metabolism” Journal of Internal Medicine 2015, IF 6.5, 80 citations
• Philippe J. and Dibner C. “Thyroid circadian timing: roles in physiology and thyroid malignancies” J Biol Rhythms 2015 Apr;30(2):76-83. IF 3.3, 15 citations.

2014

Thèses de privat-docent

Dibner C. Roles of human peripheral circadian system in metabolic diseases and cancer. 2014

2013

Articles scientifiques

• Pulimeno P, Mannic T, Sage D, Giovannoni L, Salmon P, Lemeille S, Giry-Laterriere M, Unser M, et al. Autonomous and self-sustained circadian oscillators displayed in human islet cells. Diabetologia 2013;56(3):497-507.
• Ditisheim A, Dibner C, Philippe J, Pechere A. Biological rhythms and preeclampsia. Frontiers in Endocrinology 2013;4:47.
• Mannic T, Meyer P, Triponez F, Pusztaszeri M, Le Martelot G, Mariani O, Schmitter D, Sage D, et al. Circadian clock characteristics are altered in human thyroid malignant nodules. Journal of Clinical Endocrinology and Metabolism 2013;98(11):4446-56.

Directions de master

• Perrin L. Characterization of the circadian clockwork in human skeletal muscle cells. 2013

2012

Articles scientifiques

• Gonzalez Rodriguez E, Hernandez A, Dibner C, Koehler B, Pechere A. Implications cliniques du cycle circadien de la pression artérielle. Revue médicale suisse 2012;12;8(353):1709-1715.

2011

Articles scientifiques

• Gosmain Y, Cheyssac C, Heddad Masson M, Dibner C, Philippe J. Glucagon gene expression in the endocrine pancreas: the role of the transcription factor Pax6 in α-cell differentiation, glucagon biosynthesis and secretion. Diabetes, Obesity and Metabolism 2011;13 Suppl 1:31-8.

2010

Articles scientifiques

• Sage D, Unser M, Salmon P, Dibner C. A software solution for recording circadian oscillator features in time-lapse live cell microscopy. Cell Division 2010;5:17.
• Kowalska E, Moriggi E, Bauer CR, Dibner C, Brown S. The circadian clock starts ticking at a developmentally early stage. Journal of Biological Rhythms 2010;25(6):442-449.
• Dibner C, Schibler U, Albrecht U. The mammalian circadian timing system: organization and coordination of central and peripheral clocks. Annual Review of Physiology 2010;72:517-549.
• Gutkovich YE, Ofir R, Elkouby YM, Dibner C, Gefen A, Elias S, Frank D. Xenopus Meis3 protein lies at a nexus downstream to Zic1 and Pax3 proteins, regulating multiple cell-fates during early nervous system development. Developmental Biology 2010;338(1):50-62.

Related video and links:

ECM Video 1 and 3

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563957/

http://www.jove.com/video/54673/parallel-measurement-circadian-clock-ge…

http://bigwww.epfl.ch/sage/soft/circadian/

Téléchargez le résumé des recherches menées dans le laboratoire d'endocrinologie circadienne (en anglais)

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